About this product

Product Identifiers

PublisherOxford University Press

ISBN-100199634769

ISBN-139780199634767

eBay Product ID (ePID)76579

Product Key Features

Number of Pages288 Pages

Publication NameD N A Cloning : a Practical Approachvolume 1: Core Techniques

LanguageEnglish

SubjectLife Sciences / Molecular Biology, Life Sciences / Biochemistry, Genetics

Publication Year1995

FeaturesRevised

TypeTextbook

AuthorB. D. Hames

Subject AreaScience, Medical

SeriesPractical Approach Ser.

FormatUk-Trade Paper

Dimensions

Item Height0.6 in

Item Weight17.6 Oz

Item Length9.2 in

Item Width6.1 in

Additional Product Features

Edition Number2

Intended AudienceCollege Audience

LCCN94-026740

Series Volume Number148

IllustratedYes

Table Of Content1. Techniques for transformation of E.coli2. Lambda replacement vectors in the construction of genomic DNA libraries3. Procedures for cDNA cloning4. Making probes5. Using cloned DNAs to analyse gene organization and expression6. Choices of single-stranded vectors and their application for in vitro mutagenesis7. PCR and its applications8. DNA sequencing

Edition DescriptionRevised edition

SynopsisThis book is one of four which together are considerably updated and totally revised texts that arose out of the very successful "DNA Cloning, Volume I-IV" books in the Practical Approach series. This particular book describes all the necessary background and detailed laboratory methods for the core techniques used in every molecular biology laboratory., This volume provides detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments for the steps used in most molecular biology laboratories. It covers transformation, construction of cDNA and genomic libraries, probe preparation, analysis of gene organisation and expression, in vitro mutagenesis, and DNA sequencing. The information provided is a completely up to dateaccount of each topic by established researchers. These two volumes, the first of four, are a thoroughly revised and up dated version of the original ones published in the Practial Approachseries. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories., This volume provides detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments for the steps used in most molecular biology laboratories. It covers transformation, construction of cDNA and genomic libraries, probe preparation, analysis of gene organisation and expression, in vitro mutagenesis, and DNA sequencing. The information provided is a completely up to date account of each topic by established researchers. These two volumes, the first of four, are a thoroughly revised and up dated version of the original ones published in the Practial Approach series. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories., This volume provides detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments for the steps used in most molecular biology laboratories for example transformation, construction of cDNA and genomic libraries, probe preparation, analysis of gene organization and expression, in vitro mutagenesis, and DNA sequencing. The information provided is an completely up-to-date account of each topic by established researchers. These two books, the first of four volumes are thoroughly revised and up-dated versions of the original ones published in the Practical Approach series. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories.

LC Classification NumberQH442.2.D59 1994